Difference between revisions of "Part:BBa K3866002"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K3866002 short</partinfo> | <partinfo>BBa_K3866002 short</partinfo> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction. | ||
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+ | ===Design Notes=== | ||
+ | The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning. | ||
+ | The CDS has position B3-B5. | ||
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+ | <p style="text-align: center;"> | ||
+ | [[Image: T--Thessaly--GB-AATG-GCTT.jpeg|900px|thumb|none|<i><b>Figure 1.</b>The overhangs of this part in the GoldenBraid Grammar.</i>]] | ||
− | + | ===Verification of Cloning=== | |
− | < | + | [[File:T--Thessaly--laci.png|700px|thumb|none|<i><b>Fig.3:</b>(U=Uncut C=Cut) Restriction Digestion of LacI with EcoRI + SlaI, Expected bands : 3189 +2300 + 892 bp, Positive Result:C2</i>]] |
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+ | ===Source=== | ||
+ | Synthesized by Twist Biosciences. | ||
− | + | ===Sequence and Features=== | |
− | === | + | <partinfo>BBa_K3866002 SequenceAndFeatures</partinfo> 9/6/2021 |
− | <partinfo>BBa_K3866002 | + | |
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Revision as of 11:42, 25 September 2021
ackA-Acetate kinase GB compatible with B3-B5
Usage and Biology
Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.
Design Notes
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning. The CDS has position B3-B5.
Verification of Cloning
Source
Synthesized by Twist Biosciences.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]