Difference between revisions of "Part:BBa K3885007:Design"
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+ | Galizi R, Duncan JN, Rostain W, Quinn CM, Storch M, Kushwaha M, Jaramillo A. Engineered RNA-Interacting CRISPR Guide RNAs for Genetic Sensing and Diagnostics. CRISPR J. 2020 Oct;3(5):398-408. doi: 10.1089/crispr.2020.0029. PMID: 33095053. |
Revision as of 01:01, 22 September 2021
Interacting Guide RNA(igRNA)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In our project, we add a special sequence in the 5' end of the gRNA, which consists of recognition sequence A, cis-repressed sequence and recognition sequence B. The cis-repressed sequence is complementary to the spacer, leading to the repression of the binding ability between igRNA with its DNA target. The recognition sequence A and the recognition sequence B are complementary to the interacting sequence A and interacting sequence, respectively.
Source
none
References
Galizi R, Duncan JN, Rostain W, Quinn CM, Storch M, Kushwaha M, Jaramillo A. Engineered RNA-Interacting CRISPR Guide RNAs for Genetic Sensing and Diagnostics. CRISPR J. 2020 Oct;3(5):398-408. doi: 10.1089/crispr.2020.0029. PMID: 33095053.