Difference between revisions of "Part:BBa K108024"

 
 
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<partinfo>BBa_K108024 short</partinfo>
 
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component of RuvABC resolvasome, endonuclease
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component of RuvABC resolvasome, endonuclease, of E.coli
  
 
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<partinfo>BBa_K108024 parameters</partinfo>
 
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===Information contributed by City of London UK (2021)===
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Part information is collated here to help future users of the BioBrick registry.
 +
 +
Metadata:
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*'''Group:''' City of London UK 2021
 +
*'''Author:''' Haotong Xiong
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*'''Summary:''' Added information collated from existing scientific studies
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 +
----
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RuvC is a nuclease that resolves Holliday junction intermediates in genetic recombination.
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<ref>Iwasaki, H., M. Takahagi, T. Shiba, A. Nakata, and H. Shinagawa. 1991. “Escherichia Coli RuvC Protein Is an Endonuclease That Resolves the Holliday Structure.” The EMBO Journal 10 (13): 4381–89. https://pubmed.ncbi.nlm.nih.gov/1661673/.</ref>
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It cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.
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‌<ref>Dunderdale, H. J., F. E. Benson, C. A. Parsons, G. J. Sharples, R. G. Lloyd, and S. C. West. 1991. “Formation and Resolution of Recombination Intermediates by E. Coli RecA and RuvC Proteins.” Nature 354 (6354): 506–10. https://doi.org/10.1038/354506a0.</ref>

Latest revision as of 09:10, 21 September 2021

RuvC

component of RuvABC resolvasome, endonuclease, of E.coli

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

  • Group: City of London UK 2021
  • Author: Haotong Xiong
  • Summary: Added information collated from existing scientific studies

RuvC is a nuclease that resolves Holliday junction intermediates in genetic recombination. [1]

It cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.

‌[2]
  1. ↑ Iwasaki, H., M. Takahagi, T. Shiba, A. Nakata, and H. Shinagawa. 1991. “Escherichia Coli RuvC Protein Is an Endonuclease That Resolves the Holliday Structure.” The EMBO Journal 10 (13): 4381–89. https://pubmed.ncbi.nlm.nih.gov/1661673/.
  2. ↑ Dunderdale, H. J., F. E. Benson, C. A. Parsons, G. J. Sharples, R. G. Lloyd, and S. C. West. 1991. “Formation and Resolution of Recombination Intermediates by E. Coli RecA and RuvC Proteins.” Nature 354 (6354): 506–10. https://doi.org/10.1038/354506a0.