Difference between revisions of "Part:BBa K3731002:Design"

 
(→‎References)
Line 16: Line 16:
  
 
===References===
 
===References===
 +
Kallio, P. T.; Kim, D.; Tsai, P. S.; Bailey, J. E. Intracellular expression of Vitreoscilla hemoglobin
 +
alters Escherichia coli energy metabolism under oxygen-limited conditions. The FEBS Journal
 +
1994, 219 (1-2), 201-208.
 +
Kanak L. Dikshit, Rajendra P. Dikshit, Dale A. Webster, Study of Vitreoscilla globin( vgb ) gene expression and promoter activity in E. Coli through transcriptional fusion , Nucleic Acids Research, Volume 18, Issue 14, 25 July 1990, Pages 4149–4155.

Revision as of 15:31, 14 September 2021


vgb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR-amplified CFppk1-vgb-mazE was digested with restriction enzymes Kpn I and Hind III, after which it was insert into corresponding multiple cloning sties of the broad-host-range expressing vector pBBR1MCS2.


Source

E.Coli BL21 was purchased from China Center of Industrial Culture Collection (CICC, China). For the construction of pBBR1MCS2-ppk1-vgb-mazE, genomic DNA of E.Coli BL21 was used as the template to PCR-amplify ppk1, vgb and mazE with primers.

References

Kallio, P. T.; Kim, D.; Tsai, P. S.; Bailey, J. E. Intracellular expression of Vitreoscilla hemoglobin alters Escherichia coli energy metabolism under oxygen-limited conditions. The FEBS Journal 1994, 219 (1-2), 201-208. Kanak L. Dikshit, Rajendra P. Dikshit, Dale A. Webster, Study of Vitreoscilla globin( vgb ) gene expression and promoter activity in E. Coli through transcriptional fusion , Nucleic Acids Research, Volume 18, Issue 14, 25 July 1990, Pages 4149–4155.