Difference between revisions of "Part:BBa K4035006"

 
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<partinfo>BBa_K4035006 short</partinfo>
 
<partinfo>BBa_K4035006 short</partinfo>
  
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Copper metallotionein CUP1 (BBa_M45090) is a protein responsible for copper binding protein in the yeast Saccharomyces cerevisiae. In order to improve the copper fixation efficiency in yeast, CUP1 was linked to a second sequence of the same protein. The linker is a rigid linker and is made of four successive (EAAAK) amino acid sequences.  
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This protein is made of two yeast copper metallotionein protein, CUP1 (BBa_M45090), linked together by a rigid linker made of four times the EAAAK amino acid sequence.  
  
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===Usage and Biology===
 
===Usage and Biology===
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Copper metallotionein CUP1 (BBa_M45090) is a protein responsible for copper binding protein in the yeast Saccharomyces cerevisiae. In order to increase the copper retrieval efficiency, two copies of CUP1 were linked together and expressed at the outter surface of S. cerevisiae (BBa_K4035013). The EAAAK linker is a common rigid linker.
  
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===Characterization===
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4035006 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4035006 SequenceAndFeatures</partinfo>
  

Revision as of 06:23, 13 September 2021


Dimerization of the copper metallothionein 1 : CUP1-(EAAAK)4-CUP1

This protein is made of two yeast copper metallotionein protein, CUP1 (BBa_M45090), linked together by a rigid linker made of four times the EAAAK amino acid sequence.

Usage and Biology

Copper metallotionein CUP1 (BBa_M45090) is a protein responsible for copper binding protein in the yeast Saccharomyces cerevisiae. In order to increase the copper retrieval efficiency, two copies of CUP1 were linked together and expressed at the outter surface of S. cerevisiae (BBa_K4035013). The EAAAK linker is a common rigid linker.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 408
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 408
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 408
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 408
  • 1000
    COMPATIBLE WITH RFC[1000]