Difference between revisions of "Part:BBa K4035005"

 
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<partinfo>BBa_K4035005 short</partinfo>
 
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Copper metallotionein CUP1 (BBa_M45090) is a protein responsible for copper binding protein in the yeast Saccharomyces cerevisiae. In order to improve the copper fixation efficiency in yeast, CUP1 was linked to a second sequence of the same protein. The linker is a rigid linker and is made of three successive (EAAAK) amino acid sequences.  
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This protein is made of two yeast copper metallotionein protein, CUP1 (BBa_M45090), linked together by a rigid linker made of three times the EAAAK amino acid sequence.  
  
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===Usage and Biology===
 
===Usage and Biology===
  
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Copper metallotionein CUP1 (BBa_M45090) is a protein responsible for copper binding protein in the yeast Saccharomyces cerevisiae. In order to increase the copper retrieval efficiency, two copies of CUP1 were linked together and expressed at the outter surface of S. cerevisiae (BBa_K4035012). The EAAAK linker is a common rigid linker.
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<span class='h3bb'>Sequence and Features</span>
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===Characterization===
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===Sequence and Features===
 
<partinfo>BBa_K4035005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4035005 SequenceAndFeatures</partinfo>
  

Revision as of 06:14, 13 September 2021


Dimerization of the copper metallothionein 1 : CUP1-(EAAAK)3-CUP1

This protein is made of two yeast copper metallotionein protein, CUP1 (BBa_M45090), linked together by a rigid linker made of three times the EAAAK amino acid sequence.

Usage and Biology

Copper metallotionein CUP1 (BBa_M45090) is a protein responsible for copper binding protein in the yeast Saccharomyces cerevisiae. In order to increase the copper retrieval efficiency, two copies of CUP1 were linked together and expressed at the outter surface of S. cerevisiae (BBa_K4035012). The EAAAK linker is a common rigid linker.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 384