Difference between revisions of "Part:BBa J70027:Design"
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<partinfo>BBa_J70027 short</partinfo> | <partinfo>BBa_J70027 short</partinfo> | ||
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The gene for PE contains no CGG codons. | The gene for PE contains no CGG codons. | ||
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| + | The primer names are for J70023 -- this is a shortened version | ||
GTTTCTTCGAATTCGCGGCCGCTTCTAGAGtaattataaaaacaccttattttac,J00023-F2 | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGtaattataaaaacaccttattttac,J00023-F2 | ||
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GTTTCTTCCTGCAGCGGCCGCTACTAGTAttgggagtgcggggtttggggtgc,J00023-R | GTTTCTTCCTGCAGCGGCCGCTACTAGTAttgggagtgcggggtttggggtgc,J00023-R | ||
The start of J70027 matches the origin start in the pSci1 plasmid. | The start of J70027 matches the origin start in the pSci1 plasmid. | ||
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===Source=== | ===Source=== | ||
Revision as of 18:37, 4 November 2008
Mesoplasma florum plasmid origin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 372
Design Notes
No native restriction sites. Added biobrick ends.
The gene for PE contains no CGG codons.
The primer names are for J70023 -- this is a shortened version
GTTTCTTCGAATTCGCGGCCGCTTCTAGAGtaattataaaaacaccttattttac,J00023-F2
GTTTCTTCCTGCAGCGGCCGCTACTAGTAttgggagtgcggggtttggggtgc,J00023-R
The start of J70027 matches the origin start in the pSci1 plasmid.
Source
Genbank AJ969070