Difference between revisions of "Part:BBa K079020:Experience"

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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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how you used this part and how it worked out.
 
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===Applications of BBa_K079020===
 
===Applications of BBa_K079020===
  
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K079020 is a closed-loop LacI Reporter where GFP expression is auto-regulated.
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K079020 is a closed-loop auto-regulated LacI reporter.It was transformed in HL1Blue bacterial cells according to the standard protocol. One colony from each plate was picked up and let grow overnight in LB medium at 37°C. O/N culture was spinned at 6000-8000 rpm for three minutes. The supernatant was harvested and the pellet resuspended. Slides were prepared for the fluorescence bacteria image acquisition. For each slide five different views were acquired. Finally, images were elaborated with the Visual Fluo Bacteria Software. Examples of fluorescence bacteria image are shown in the following figure. The fluorescence images reveal the repression due to the presence of the Lac operator compared to K079020 (open loop circuit).  
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It was transformed in HL1Blue bacterial cells according to the standard protocol. One colony from each plate was picked up and let grow overnight in LB medium at 37°C. O/N culture was spinned at 6000-8000 rpm for three minutes. The supernatant was harvested and the pellet resuspended. Slides were prepared for the fluorescence bacteria image acquisition. For each slide five different views were acquired. Finally, images were elaborated with the Visual Fluo Bacteria Software. Examples of fluorescence bacteria image are shown in the following figure. The fluorescence images reveal the repression due to the presence of the Lac operator compared to K079020 (open loop circuit).  
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[[Image:ClosedLooop.jpg |500 px|center |thumbnail| K079020 Fluorescence Image from O/N culture]]
  
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[[Image:ClosedLooop.jpg | px 300|center |thumbnail| K079020 Fluorescence Image]]
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[[Image:Table_Closed.jpg | px 300|center |thumbnail| K079020 Fluorescence Image]]
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The analysis result by Visual Fluo Bacteria is reported in the following table:
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[[Image:Table_Closed_.jpg |900 px|center |thumbnail| K079020 Fluorescence Image]]
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 14:53, 4 November 2008

Applications of BBa_K079020

K079020 is a closed-loop auto-regulated LacI reporter.It was transformed in HL1Blue bacterial cells according to the standard protocol. One colony from each plate was picked up and let grow overnight in LB medium at 37°C. O/N culture was spinned at 6000-8000 rpm for three minutes. The supernatant was harvested and the pellet resuspended. Slides were prepared for the fluorescence bacteria image acquisition. For each slide five different views were acquired. Finally, images were elaborated with the Visual Fluo Bacteria Software. Examples of fluorescence bacteria image are shown in the following figure. The fluorescence images reveal the repression due to the presence of the Lac operator compared to K079020 (open loop circuit).

K079020 Fluorescence Image from O/N culture


The analysis result by Visual Fluo Bacteria is reported in the following table:

K079020 Fluorescence Image

User Reviews

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