Difference between revisions of "Part:BBa K4033005"

 
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<partinfo>BBa_K4033005 short</partinfo>
  
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<i>Ant B</i> is extracted from Photorhabdus luminescens, and is responsible for charging Ant F which functionalizes Ant F with a 4’ phosphopantetheine arm necessary for activity.[1]
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<b><font size="3">Biology and Usage</font></b>
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Ant B and Coenzyme A (CoA) ligase (Ant G), were required as alternative auxiliary enzymes to carry out the posttranslation modification - functionalize ACP (Ant F) with a 4’ phosphopantetheine arm necessary for the activity (Ant F is charged by Ant B)– which resulted in detectable biosynthesis of molecules putatively identified as SEK4, SEK4b. E. coli endogenous 4’ phosphopantetheinyl transferases (PPTases)—AcpS and Ent D—could not efficiently realize this process. Expression of the mPKS (antDEF) alone did not produce any detectable masses corresponding to SEK4/SEK4b.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4033005 SequenceAndFeatures</partinfo>
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<b><font size="3">Experimental approach</font></b>
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<b><font size="3">References</font></b>
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[1] Cummings M, Peters AD, Whitehead GFS, Menon BRK, Micklefield J, Webb SJ, et al. (2019) Assembling a plug-and-play production line for combinatorial biosynthesis of aromatic polyketides in Escherichia coli. PLoS Biol 17(7): e3000347. https://doi.org/10.1371/journal.pbio.3000347

Revision as of 14:20, 29 August 2021

Ant B

Ant B is extracted from Photorhabdus luminescens, and is responsible for charging Ant F which functionalizes Ant F with a 4’ phosphopantetheine arm necessary for activity.[1]


Biology and Usage

Ant B and Coenzyme A (CoA) ligase (Ant G), were required as alternative auxiliary enzymes to carry out the posttranslation modification - functionalize ACP (Ant F) with a 4’ phosphopantetheine arm necessary for the activity (Ant F is charged by Ant B)– which resulted in detectable biosynthesis of molecules putatively identified as SEK4, SEK4b. E. coli endogenous 4’ phosphopantetheinyl transferases (PPTases)—AcpS and Ent D—could not efficiently realize this process. Expression of the mPKS (antDEF) alone did not produce any detectable masses corresponding to SEK4/SEK4b.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental approach



References

[1] Cummings M, Peters AD, Whitehead GFS, Menon BRK, Micklefield J, Webb SJ, et al. (2019) Assembling a plug-and-play production line for combinatorial biosynthesis of aromatic polyketides in Escherichia coli. PLoS Biol 17(7): e3000347. https://doi.org/10.1371/journal.pbio.3000347