Difference between revisions of "Part:BBa K3728002"
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Revision as of 04:13, 24 August 2021


pTol2: a Tol2 vector - the Tol2 transposable element

T--Mingdao--BBa K3728002-1.png
<img src="T--Mingdao--BBa_K3728002-1.png" width="90%" />

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Expression

The fluorescence expression levels were measured for lactate dehydrogenase promoter (ldhp) [ldhp-GFP-Tr/pSB1C3 (BBa_K3376002)] and thiol peroxidase promoter (tpxp) [tpxp-GFP-Tr/pSB1C3 (BBa_K3376004)] activity of S. mutans. GFP was detected at Ex/Em = 483/513. As shown in the figure, the expression level of ldhp-driven GFP was very high compared to a regulated promoter (tpxp) and a vector-only control.






Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3452
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 29
    Illegal NotI site found at 3458
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3452
    Illegal BglII site found at 3340
    Illegal XhoI site found at 3419
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3452
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3452
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3467
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 642
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1818
    Illegal SapI.rc site found at 2900