Difference between revisions of "Part:BBa K3941001:Design"

 
 
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===Design Notes===
 
===Design Notes===
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This part is created from only the coding sequence of Trichoderma reesei endoglucanase II gene by excluding introns and non-coding exon sites. Signal sequence is also removed.
+
This part is created from only the coding sequence of Trichoderma reesei endoglucanase II gene by excluding introns and non-coding exon sites. Signal sequence is also removed. Codon optimization has carried out to increase production in Escherichia coli bacterium. His-tag has added to 3' end of the sequence to help purification of protein product.
  
  
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===Source===
 
===Source===
  
−
The source of this pars is Trichoderma reesei endoglucanase II gene.
+
The source of this part is Trichoderma reesei endoglucanase II gene.
  
 
===References===
 
===References===

Latest revision as of 11:51, 1 August 2021


EGII


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is created from only the coding sequence of Trichoderma reesei endoglucanase II gene by excluding introns and non-coding exon sites. Signal sequence is also removed. Codon optimization has carried out to increase production in Escherichia coli bacterium. His-tag has added to 3' end of the sequence to help purification of protein product.


Source

The source of this part is Trichoderma reesei endoglucanase II gene.

References