Difference between revisions of "Part:BBa K1500994"
(→‎Information contributed by City of London UK (2021))
 
(2 intermediate revisions by the same user not shown)
Line 8: Line 8:
 
Part information is collated here to help future users of the BioBrick registry.
 
Part information is collated here to help future users of the BioBrick registry.
  
−
dam causes DNA methylation within the sequence GATC, preventing cleavage by the MboI restriction endonuclease [https://pubmed.ncbi.nlm.nih.gov/368070]
+
Metadata:
 +
*'''Group:''' City of London UK 2021
 +
*'''Author:''' Lucas Ng
 +
*'''Summary:''' Added information collated from existing scientific studies
  
−
It is likely to act in postreplication mismatch repair [https://pubmed.ncbi.nlm.nih.gov/6308634] rather than as a member of a restriction modification system, despite sharing sequence specificity with several type II restriction methylases and endonucleases as part of the N(4)/N(6)-methyltransferase family [https://pubmed.ncbi.nlm.nih.gov/25845600]
+
----
  
−
Furthermore, it may also be involved in DNA replication [https://pubmed.ncbi.nlm.nih.gov/3891329]
+
dam causes DNA methylation within the sequence GATC, preventing cleavage by the MboI restriction endonuclease
 +
<ref>Geier, G. E., and P. Modrich. 1979. “Recognition Sequence of the Dam Methylase of Escherichia Coli K12 and Mode of Cleavage of Dpn I Endonuclease.” The Journal of Biological Chemistry 254 (4): 1408–13. https://pubmed.ncbi.nlm.nih.gov/368070.</ref>.
  
−
====Reaction====
+
It is likely to act in postreplication mismatch repair
−
a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine
+
<ref>Lu, A. L., S. Clark, and P. Modrich. 1983. “Methyl-Directed Repair of DNA Base-Pair Mismatches in Vitro.” Proceedings of the National Academy of Sciences 80 (15): 4639–43. https://doi.org/10.1073/pnas.80.15.4639.</ref>
 +
rather than as a member of a restriction modification system, despite sharing sequence specificity with several type II restriction methylases and endonucleases as part of the N(4)/N(6)-methyltransferase family
 +
<ref>Horton, John R., Xing Zhang, Robert M. Blumenthal, and Xiaodong Cheng. 2015. “Structures of Escherichia Coli DNA Adenine Methyltransferase (Dam) in Complex with a Non-GATC Sequence: Potential Implications for Methylation-Independent Transcriptional Repression.” Nucleic Acids Research 43 (8): 4296–4308. https://doi.org/10.1093/nar/gkv251.</ref>.
  
−
[https://www.rhea-db.org/rhea/15197]
+
Furthermore, it may also be involved in DNA replication
 +
<ref>Smith, D. W., A. M. Garland, G. Herman, R. E. Enns, T. A. Baker, and J. W. Zyskind. 1985. “Importance of State of Methylation of OriC GATC Sites in Initiation of DNA Replication in Escherichia Coli.” The EMBO Journal 4 (5): 1319–26. https://pubmed.ncbi.nlm.nih.gov/3891329.</ref>
  
 +
====Reaction====
 +
a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine
 +
<ref>“Rhea - Annotated Reactions Database.” n.d. Rhea. Accessed July 26, 2021. https://www.rhea-db.org/rhea/15197.</ref>
  
−
<!-- Uncomment this to enable Functional Parameter display
+
===References===
−
===Functional Parameters===
+
−
<partinfo>BBa_K1500994 parameters</partinfo>
+
−
<!-- -->
+

Latest revision as of 17:37, 26 July 2021


Dam

Involved in methylating DNA, overexpression causes 6.4 * 10^-4 /// 6.4 * 10^4 mutation rate increase, mostly catalyzing transversions.

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

  • Group: City of London UK 2021
  • Author: Lucas Ng
  • Summary: Added information collated from existing scientific studies

dam causes DNA methylation within the sequence GATC, preventing cleavage by the MboI restriction endonuclease [1].

It is likely to act in postreplication mismatch repair [2] rather than as a member of a restriction modification system, despite sharing sequence specificity with several type II restriction methylases and endonucleases as part of the N(4)/N(6)-methyltransferase family [3].

Furthermore, it may also be involved in DNA replication [4]

Reaction

a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine [5]

References

  1. ↑ Geier, G. E., and P. Modrich. 1979. “Recognition Sequence of the Dam Methylase of Escherichia Coli K12 and Mode of Cleavage of Dpn I Endonuclease.” The Journal of Biological Chemistry 254 (4): 1408–13. https://pubmed.ncbi.nlm.nih.gov/368070.
  2. ↑ Lu, A. L., S. Clark, and P. Modrich. 1983. “Methyl-Directed Repair of DNA Base-Pair Mismatches in Vitro.” Proceedings of the National Academy of Sciences 80 (15): 4639–43. https://doi.org/10.1073/pnas.80.15.4639.
  3. ↑ Horton, John R., Xing Zhang, Robert M. Blumenthal, and Xiaodong Cheng. 2015. “Structures of Escherichia Coli DNA Adenine Methyltransferase (Dam) in Complex with a Non-GATC Sequence: Potential Implications for Methylation-Independent Transcriptional Repression.” Nucleic Acids Research 43 (8): 4296–4308. https://doi.org/10.1093/nar/gkv251.
  4. ↑ Smith, D. W., A. M. Garland, G. Herman, R. E. Enns, T. A. Baker, and J. W. Zyskind. 1985. “Importance of State of Methylation of OriC GATC Sites in Initiation of DNA Replication in Escherichia Coli.” The EMBO Journal 4 (5): 1319–26. https://pubmed.ncbi.nlm.nih.gov/3891329.
  5. ↑ “Rhea - Annotated Reactions Database.” n.d. Rhea. Accessed July 26, 2021. https://www.rhea-db.org/rhea/15197.