Difference between revisions of "Part:BBa K108020"

(Information contributed by City of London UK (2021))
(Information contributed by City of London UK (2021))
Line 11: Line 11:
 
*'''Author:''' Lucas Ng
 
*'''Author:''' Lucas Ng
 
*'''Summary:''' Added information collated from existing scientific studies
 
*'''Summary:''' Added information collated from existing scientific studies
 +
 +
----
  
 
dam causes DNA methylation within the sequence GATC, preventing cleavage by the MboI restriction endonuclease [https://pubmed.ncbi.nlm.nih.gov/368070]
 
dam causes DNA methylation within the sequence GATC, preventing cleavage by the MboI restriction endonuclease [https://pubmed.ncbi.nlm.nih.gov/368070]

Revision as of 10:56, 26 July 2021

Dam

DNA adenine methylase of E.coli

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

  • Group: City of London UK 2021
  • Author: Lucas Ng
  • Summary: Added information collated from existing scientific studies

dam causes DNA methylation within the sequence GATC, preventing cleavage by the MboI restriction endonuclease [1]

It is likely to act in postreplication mismatch repair [2] rather than as a member of a restriction modification system, despite sharing sequence specificity with several type II restriction methylases and endonucleases as part of the N(4)/N(6)-methyltransferase family [3]

Furthermore, it may also be involved in DNA replication [4]

Reaction

a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine

[5]