Difference between revisions of "Part:BBa K3767001"
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<partinfo>BBa_K3767001 short</partinfo> | <partinfo>BBa_K3767001 short</partinfo> | ||
| − | Alkaline phosphatases are a group of isoenzymes that catalyze the hydrolysis of organic phosphate esters present in the extracellular space | + | Alkaline phosphatases are a group of isoenzymes that catalyze the hydrolysis of organic phosphate esters present in the extracellular space [[Part:BBa_K3767001#References|<sup>[1]</sup>]] . This catalytic activity is commonly used in diagnostic tests as the cleavage of the phosphate ester produces fluorescence visible to the naked eye. [[File:BBa K3767001 Coloured PhoA x40.png|thumb| 3D representation of the alkaline phosphatase]] |
Revision as of 16:16, 11 June 2021
Alkaline Phosphatase optimized for E. Coli w/ 40x catalytic activity
Usage and Biology
Alkaline phosphatases are used as reporter enzymes in different assays such as Western Blotting and in situ hybridization[1]. Testing human blood for Alkaline Phosphatase levels is a routine test that can reveal different conditions[2].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
Description