Difference between revisions of "Part:BBa K3767001"
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===Usage and Biology===
 
===Usage and Biology===
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Alkaline phosphatases are used as reporter enzymes in different assays such as Western Blotting and in situ hybridization[[Part:BBa_K1216001#References|<sup>[1]</sup>]]. Testing human blood for Alkaline Phosphatase levels is a routine test that can reveal different conditions[[Part:BBa_K1216001#References|<sup>[2]</sup>]].
  
 
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Revision as of 14:22, 11 June 2021


Alkaline Phosphatase optimized for E. Coli w/ 40x catalytic activity

Alkaline phosphatases are a group of isoenzymes that catalyze the hydrolysis of organic phosphate esters present in the extracellular space (1) [1] . This catalytic activity is commonly used in diagnostic tests as the cleavage of the phosphate ester produces fluorescence visible to the naked eye.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]