Difference between revisions of "Part:BBa K3416005"
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5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (EC 3.2.2.9) (MTAN) catalyzes the irreversible cleavage of the glycosidic bond in 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH) and plays a key role in four metabolic processes: biological methylation, polyamine biosynthesis, methionine recycling and bacterial quorum sensing. | 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (EC 3.2.2.9) (MTAN) catalyzes the irreversible cleavage of the glycosidic bond in 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH) and plays a key role in four metabolic processes: biological methylation, polyamine biosynthesis, methionine recycling and bacterial quorum sensing. | ||
H<sub>2</sub>O + S-adenosyl-L-homocysteine → adenine + S-(5-deoxy-D-ribose-5-yl)-L-homocysteine | H<sub>2</sub>O + S-adenosyl-L-homocysteine → adenine + S-(5-deoxy-D-ribose-5-yl)-L-homocysteine | ||
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| + | =Introduction= | ||
| + | [[File:T--Vilnius-Lithuania--FFlogo.png|100px|right|FlavoFlow]] | ||
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| + | Vilnius-Lithuania iGEM 2020 project [https://2020.igem.org/Team:Vilnius-Lithuania <b>FlavoFlow]</b>includes three goals towards looking for <i>Flavobacterium</i> disease-related problems’ solutions. The project includes creating a rapid detection kit, based on HDA and LFA, developing an implement for treating a disease, and introducing the foundation of edible vaccines. | ||
| + | This part was used for the second goal- treatment - of the project FlavoFlow. | ||
| + | __TOC__ | ||
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| + | =Biology= | ||
| + | ===Description of the Pfs=== | ||
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| + | 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (EC 3.2.2.9) cleaves the glycosidic bond of 5’-methylthioadenosine (MTA) and S-adenosylhomocysteine (AdoHcy) to adenine and its corresponding thiol ribose. This hydrolysis reaction is irreversible, with Km=0.43μM and 4.3μM for MTA and AdoHcy, respectively<ref name ="Second">Lee, J. E., Cornell, K. A., Riscoe, M. K. & Howell, P. L. Structure of E. coli 5'-methylthioadenosine/S-adenosylhomocysteine Nucleosidase Reveals Similarity to the Purine Nucleoside Phosphorylases. ''Structure'' '''9''', 941–953 (2001).</ref>. MTA and AdoHcy are catabolized differently in mammals and microbes<ref>Lee, J. E. et al. Mutational Analysis of a Nucleosidase Involved in Quorum-Sensing Autoinducer-2 Biosynthesis. ''Biochemistry'' '''44''', 11049–11057 (2005).</ref>. | ||
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| + | The MTA/AdoHcy nucleosidase active has three separate regions, the purine, ribose, and 5'-alkylthio binding subsites. The three region active site was determined by the presence of adenine and the inhibitors: 5'-methylthio tubercidin (MTT), formycin A (FMA), 5'-methylthio-immucillin A (MT-ImmA), and 5'-methylthio-4'- deoxy-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin A (MTDADMe-ImmA). The structure of MTA/AdoHcy nucleosidase suggests that enzyme is functional as a dimer, with each monomer consisting of a nine-stranded mixed β sheet flanked by six α helices and a small 3<sub>10</sub> helix. The nucleosidase has no similarity to any known protein<ref name ="Second">2</ref>. | ||
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| + | <span class='h3bb'>Sequence and Features</span> | ||
| + | <partinfo>BBa_K3416005 SequenceAndFeatures</partinfo> | ||
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| + | ===Functional Parameters=== | ||
| + | <partinfo>BBa_K3416005 parameters</partinfo> | ||
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| − | + | =References= | |
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Latest revision as of 22:13, 16 December 2020
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase/Pfs
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (EC 3.2.2.9) (MTAN) catalyzes the irreversible cleavage of the glycosidic bond in 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH) and plays a key role in four metabolic processes: biological methylation, polyamine biosynthesis, methionine recycling and bacterial quorum sensing.
H2O + S-adenosyl-L-homocysteine → adenine + S-(5-deoxy-D-ribose-5-yl)-L-homocysteine
Introduction
Vilnius-Lithuania iGEM 2020 project FlavoFlowincludes three goals towards looking for Flavobacterium disease-related problems’ solutions. The project includes creating a rapid detection kit, based on HDA and LFA, developing an implement for treating a disease, and introducing the foundation of edible vaccines. This part was used for the second goal- treatment - of the project FlavoFlow.
Biology
Description of the Pfs
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (EC 3.2.2.9) cleaves the glycosidic bond of 5’-methylthioadenosine (MTA) and S-adenosylhomocysteine (AdoHcy) to adenine and its corresponding thiol ribose. This hydrolysis reaction is irreversible, with Km=0.43μM and 4.3μM for MTA and AdoHcy, respectively[1]. MTA and AdoHcy are catabolized differently in mammals and microbes[2].
The MTA/AdoHcy nucleosidase active has three separate regions, the purine, ribose, and 5'-alkylthio binding subsites. The three region active site was determined by the presence of adenine and the inhibitors: 5'-methylthio tubercidin (MTT), formycin A (FMA), 5'-methylthio-immucillin A (MT-ImmA), and 5'-methylthio-4'- deoxy-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin A (MTDADMe-ImmA). The structure of MTA/AdoHcy nucleosidase suggests that enzyme is functional as a dimer, with each monomer consisting of a nine-stranded mixed β sheet flanked by six α helices and a small 310 helix. The nucleosidase has no similarity to any known protein[1].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 223
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ 1.0 1.1 Lee, J. E., Cornell, K. A., Riscoe, M. K. & Howell, P. L. Structure of E. coli 5'-methylthioadenosine/S-adenosylhomocysteine Nucleosidase Reveals Similarity to the Purine Nucleoside Phosphorylases. Structure 9, 941–953 (2001).
- ↑ Lee, J. E. et al. Mutational Analysis of a Nucleosidase Involved in Quorum-Sensing Autoinducer-2 Biosynthesis. Biochemistry 44, 11049–11057 (2005).