Difference between revisions of "Part:BBa K3513006"
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=='''Improved by NFLS 2020'''==
 
=='''Improved by NFLS 2020'''==
  
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=='''Improved by NFLS 2020'''==
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===Biology===
  
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<p>Part improvement by NFLS 2020</p>
+
<p>NF-κB is one of the most pathways inside the human body. This part contains five repeats of 5′-GGGTTTCCCC-3′as NF-κB binding site and a minimal CMV promoter. The binding sites repeats is derived from gene vascular cell adhesion molecule 1, VCAM1. NF-κB is the down-stream of TNF signal, so in the experiments we add TNF to induce NF-κB production in cells. </p>
  
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<p>Team NFLS improved the efficiency of this part by designing a new part called JTi1 consists of five repeats of 5′-GGGTTTCCCC-3′as NF-κB binding site and a minimal CMV promoter. The binding sites repeats is derived from gene vascular cell adhesion molecule 1, VCAM1. This new reconstructed promoter is more than twice as efficient as this original part.</p>
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===Usage===
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<p>We added a Secreted Alkaline Phosphatase (SEAP) which commonly used as a reporter gene behind the promoter. The enzyme activity of SEAP reflects the efficacy of the promoter. </p>
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<p>The improved part is: <a href="https://parts.igem.org/Part: BBa_K3513006">BBa_K3513006</a></p>
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<p>This part is used as a part improvement of BBa_K2976009.</p>
 +
<p>Our new part we designed here improved the efficiency more than twice than the original part. We added a Secreted Alkaline Phosphatase (SEAP) which commonly used as a reporter gene behind the promoter. The enzyme activity of SEAP reflects the efficacy of the promoter. </p>
 +
<p>The origin part is: [https://parts.igem.org/Part:BBa_K2976009'''BBa_K2976009''']</p>
  
 
[[File:T--NFLS--p1.jpg|600px|thumb|center|'''''']]
 
[[File:T--NFLS--p1.jpg|600px|thumb|center|'''''']]

Latest revision as of 12:37, 9 December 2020


JTi1

Promoter JTi2 consists of five repeats of 5′-GGGTTTCCCC-3′as NF-κB binding site and a minimal CMV promoter. The binding sites repeats is derived from gene vascular cell adhesion molecule 1, VCAM1. The reconstructed promoter is more than twice as efficient as the original part BBa_K2976009.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Improved by NFLS 2020

Biology

NF-κB is one of the most pathways inside the human body. This part contains five repeats of 5′-GGGTTTCCCC-3′as NF-κB binding site and a minimal CMV promoter. The binding sites repeats is derived from gene vascular cell adhesion molecule 1, VCAM1. NF-κB is the down-stream of TNF signal, so in the experiments we add TNF to induce NF-κB production in cells.

Usage

This part is used as a part improvement of BBa_K2976009.

Our new part we designed here improved the efficiency more than twice than the original part. We added a Secreted Alkaline Phosphatase (SEAP) which commonly used as a reporter gene behind the promoter. The enzyme activity of SEAP reflects the efficacy of the promoter.

The origin part is: BBa_K2976009

'

The standard curve:

'




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]