Difference between revisions of "Part:BBa K3606809"

Latest revision as of 02:43, 5 December 2020

mcbD

McbD works as synthase responsible for the maturation of MccB17.

Design:

Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbD into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbD expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbD product was produced.

Results:

More information：https://parts.igem.org/Part:BBa_K3606814

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 374
Illegal PstI site found at 407
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 374
Illegal PstI site found at 407
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 281
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 374
Illegal PstI site found at 407
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 374
Illegal PstI site found at 407
Illegal NgoMIV site found at 36
Illegal AgeI site found at 208
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3606809 short</partinfo>
-tba
+McbD works as synthase responsible for the maturation of MccB17.
+<h2>Design:</h2>
+Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbD into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbD expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbD product was produced.
+<h2>Results:</h2>
+More information：https://parts.igem.org/Part:BBa_K3606814
 <!-- Add more about the biology of this part here