Difference between revisions of "Part:BBa K3606807"
 
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McbB works as synthase responsible for the maturation of MccB17.
 
McbB works as synthase responsible for the maturation of MccB17.
 
<h2>Design:</h2>
 
<h2>Design:</h2>
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Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbB into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbB expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbA product was produced.
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Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbB into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbB expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbB product was produced.
  
 
<h2>Results:</h2>
 
<h2>Results:</h2>
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https://2020.igem.org/wiki/images/2/22/T--Fudan--img_McbA-E-G-BCD.jpeg
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More information：https://parts.igem.org/Part:BBa_K3606814
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Figure 1. SDS-PAGE result. lane 1: mcbA  lane 2: mcbA+IPTG lane 3: mcbE  lane 4: mcbE+IPTG  lane 5: mcbG  lane 6: mcbG+IPTG  lane 7: mcbBCD  lane 8: mcbBCD+IPTG lane 9: pGEX+ IPTG
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Comparing the 1th and 2th lanes, the difference is small. This may be due to the low expression or low molecular weight of McbA products.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:42, 5 December 2020


mcbB

Usage and Biology:

McbB works as synthase responsible for the maturation of MccB17.

Design:

Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbB into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbB expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbB product was produced.

Results:

More information：https://parts.igem.org/Part:BBa_K3606814

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]