Difference between revisions of "Part:BBa K3606813"
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<partinfo>BBa_K3606813 short</partinfo> | <partinfo>BBa_K3606813 short</partinfo> | ||
| − | + | This part is an antibiotic coding gene cluster with proteins for MccB17 maturation. | |
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| + | <h2>Design:</h2> | ||
| + | Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we wanted to know whether one promoter can start two consecutive genes. We successfully cloned McbBC into the pGEX plasmid together downstream one promoter and transcribed it into BL21. After 5 hours of induction of McbBC expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbBC product was produced. | ||
| + | |||
| + | <h2>Results:</h2> | ||
| + | More information：https://parts.igem.org/Part:BBa_K3606814 | ||
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Latest revision as of 02:41, 5 December 2020
mcbBC
This part is an antibiotic coding gene cluster with proteins for MccB17 maturation.
Design:
Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we wanted to know whether one promoter can start two consecutive genes. We successfully cloned McbBC into the pGEX plasmid together downstream one promoter and transcribed it into BL21. After 5 hours of induction of McbBC expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbBC product was produced.
Results:
More information：https://parts.igem.org/Part:BBa_K3606814
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]