Difference between revisions of "Part:BBa K3606812"
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<partinfo>BBa_K3606812 short</partinfo> | <partinfo>BBa_K3606812 short</partinfo> | ||
| − | + | <h2>Usage and Biology:</h2> | |
| + | McbG is responsible for immunity against MccB17. | ||
| + | |||
| + | <h2>Design:</h2> | ||
| + | Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbG into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbG expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbG product was produced, yet the result of colony PCR turns out as false positive. | ||
| + | |||
| + | <h2>Results:</h2> | ||
| + | |||
| + | We've constructed the plasmid for mcbG expression, yet the results of colony PCR is false positive thus further experiment is needed for the fundamental validation of the antimicrobial peptide(mccb17) expression. | ||
| + | https://2020.igem.org/wiki/images/f/f3/T--Fudan--Poster_Mcb-gels.jpg | ||
| + | Figure2. gel map for mcbA, mcbC, mcbE and mcbF | ||
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Latest revision as of 02:40, 5 December 2020
mcbG
Usage and Biology:
McbG is responsible for immunity against MccB17.
Design:
Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbG into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbG expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbG product was produced, yet the result of colony PCR turns out as false positive.
Results:
We've constructed the plasmid for mcbG expression, yet the results of colony PCR is false positive thus further experiment is needed for the fundamental validation of the antimicrobial peptide(mccb17) expression.
Figure2. gel map for mcbA, mcbC, mcbE and mcbF
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]