Difference between revisions of "Part:BBa K3513006"

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	===Functional Parameters===		===Functional Parameters===
	<partinfo>BBa_K3513006 parameters</partinfo>		<partinfo>BBa_K3513006 parameters</partinfo>
		+	<!-- -->
		+	=='''Improved by NFLS 2020'''==
		+
		+	=='''Improved by NFLS 2020'''==
		+
		+	<p>Part improvement by NFLS 2020</p>
		+
		+	<p>Team NFLS improved the efficiency of this part by designing a new part called JTi1 consists of five repeats of 5′-GGGTTTCCCC-3′as NF-κB binding site and a minimal CMV promoter. The binding sites repeats is derived from gene vascular cell adhesion molecule 1, VCAM1. This new reconstructed promoter is more than twice as efficient as this original part.</p>
		+	<p>We added a Secreted Alkaline Phosphatase (SEAP) which commonly used as a reporter gene behind the promoter. The enzyme activity of SEAP reflects the efficacy of the promoter. </p>
		+	<p>The improved part is: <a href="https://parts.igem.org/Part: BBa_K3513006">BBa_K3513006</a></p>
		+
		+	[[File:T--NFLS--p1.jpg|600px|thumb|center|'''''']]
		+	<p>The standard curve: </p>
		+
		+	[[File:T--NFLS--p2.jpg|600px|thumb|center|'''''']]
		+
		+
		+
		+
		+	<p></p>
		+
		+
		+	<!-- -->
		+	<span class='h3bb'>Sequence and Features</span>
		+	<partinfo>BBa_K2976009 SequenceAndFeatures</partinfo>
		+
		+
		+	<!-- Uncomment this to enable Functional Parameter display
		+	===Functional Parameters===
		+	<partinfo>BBa_K2976009 parameters</partinfo>
	<!-- -->		<!-- -->

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Revision as of 03:59, 28 October 2020

JTi1

Promoter JTi2 consists of five repeats of 5′-GGGTTTCCCC-3′as NF-κB binding site and a minimal CMV promoter. The binding sites repeats is derived from gene vascular cell adhesion molecule 1, VCAM1. The reconstructed promoter is more than twice as efficient as the original part BBa_K2976009.

Sequence and Features

Improved by NFLS 2020

Improved by NFLS 2020

Part improvement by NFLS 2020

Team NFLS improved the efficiency of this part by designing a new part called JTi1 consists of five repeats of 5′-GGGTTTCCCC-3′as NF-κB binding site and a minimal CMV promoter. The binding sites repeats is derived from gene vascular cell adhesion molecule 1, VCAM1. This new reconstructed promoter is more than twice as efficient as this original part.

We added a Secreted Alkaline Phosphatase (SEAP) which commonly used as a reporter gene behind the promoter. The enzyme activity of SEAP reflects the efficacy of the promoter.

The improved part is: <a href="https://parts.igem.org/Part: BBa_K3513006">BBa_K3513006</a>

The standard curve:

Sequence and Features