Difference between revisions of "Part:BBa K3657016"
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This part is a modification of the original Broccoli aptamer. It was re-engineered to exclude the BsmbI cutting site.
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This part is a modification of the original Broccoli aptamer (<a href="https://parts.igem.org/Part:BBa_K1713020">BBa_K1713020</a>). It was re-engineered to exclude the BsmbI cutting site.
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===Usage and Biology===
 
===Usage and Biology===
 
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Broccoli is an aptamer that forms a stable secondary structure and binds the fluorogen DFHBI, which results in green fluorescence. The absence of BsmbI site allows cloning of this part with <a href="http://2018.igem.org/Team:Marburg/Part_Collection">Marburg Collection protocols</a>.
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Broccoli is an aptamer that forms a stable secondary structure and binds the fluorogen DFHBI, which results in green fluorescence. The absence of BsmbI site allows cloning of this part with <a href="http://2018.igem.org/Team:Marburg/Part_Collection">Marburg Collection system</a>.
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<p>Due to the COVID pandemic we were not able to experimentally prove that this Broccoli modification binds DFHBI correctly <i>in vitro</i>.</p>
 
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<img style="width:20%" src="https://static.igem.org/mediawiki/parts/1/18/T--Heidelberg--Parts-BBa_K3657016.jpg">
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<p><b>Figure 1:</b> Secondary structure of the part.</p>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3657016 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3657016 SequenceAndFeatures</partinfo>

Latest revision as of 03:46, 28 October 2020


Broccoli Aptamer without BsmbI site
This part is a modification of the original Broccoli aptamer (BBa_K1713020). It was re-engineered to exclude the BsmbI cutting site.

Usage and Biology

Broccoli is an aptamer that forms a stable secondary structure and binds the fluorogen DFHBI, which results in green fluorescence. The absence of BsmbI site allows cloning of this part with Marburg Collection system.

Due to the COVID pandemic we were not able to experimentally prove that this Broccoli modification binds DFHBI correctly in vitro.

Figure 1: Secondary structure of the part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]