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===Functional Parameters===
 
===Functional Parameters===
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Latest revision as of 03:35, 28 October 2020


Lys2-YCF1pr-mScarleti cassette

This cassette is the product of a two part (BBa_K3600008, BBa_K3600012) Golden Gate BsmBI assembly.

Usage and Biology

This cassette will be integrated into the yeast genome to form a reporter strain that produces a fluorescent signal when the yeast cell is under environmental stress.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1027
    Illegal EcoRI site found at 1272
    Illegal EcoRI site found at 1852
    Illegal XbaI site found at 972
    Illegal XbaI site found at 1281
    Illegal XbaI site found at 2029
    Illegal SpeI site found at 2868
    Illegal PstI site found at 2877
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1027
    Illegal EcoRI site found at 1272
    Illegal EcoRI site found at 1852
    Illegal SpeI site found at 2868
    Illegal PstI site found at 2877
    Illegal NotI site found at 756
    Illegal NotI site found at 3432
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2599
    Illegal EcoRI site found at 1027
    Illegal EcoRI site found at 1272
    Illegal EcoRI site found at 1852
    Illegal BglII site found at 1467
    Illegal BglII site found at 2470
    Illegal BglII site found at 2892
    Illegal BamHI site found at 2904
    Illegal XhoI site found at 2913
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1027
    Illegal EcoRI site found at 1272
    Illegal EcoRI site found at 1852
    Illegal XbaI site found at 972
    Illegal XbaI site found at 1281
    Illegal XbaI site found at 2029
    Illegal SpeI site found at 2868
    Illegal PstI site found at 2877
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1027
    Illegal EcoRI site found at 1272
    Illegal EcoRI site found at 1852
    Illegal XbaI site found at 972
    Illegal XbaI site found at 1281
    Illegal XbaI site found at 2029
    Illegal SpeI site found at 2868
    Illegal PstI site found at 2877
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 789