Difference between revisions of "Part:BBa K3657013"

Revision as of 03:34, 28 October 2020

RNA binding Pentatricopeptide Repeat Protein - Motif GUAGAG

This Part is an example of a PPR Protein, assembled with the PPR Domains that are specific for the four RNA bases.

It is an OFF target PPR Protein for plasmides, containing the RBS BBa_B0032 because it binds to the RNA sequence GUAGAG, which is not found in the RBS. It is an ON target PPR Protein the broccoli aptamer because it binds to the RNA sequence ACACAG, which can be find in the loop or stem region of the broccoli aptamer.
For more information, visit iGEM Heidelberg 2020

Usage and Biology

PPRs are originally found in Arabidopsis thaliana and can bind RNA. In chloroplasts, they take part in many post-translational regulation processes.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 11: / Line 11: @@
 Cytosine:<a href="https://parts.igem.org/Part:BBa_K3657009">BBa_K3657009</a><p>
-It is an ON Target PPR Protein, binding the RNA sequence GUAGAG in the loop or stem region of the broccoli aptamer.
+It is an OFF target PPR Protein for plasmides, containing the RBS <a href="https://parts.igem.org/Part:BBa_B0032">BBa_B0032</a>  because it binds to the RNA sequence GUAGAG, which is not found in the RBS.
+It is an ON target PPR Protein the broccoli aptamer because it binds to the RNA sequence ACACAG, which can be find in the loop or stem region of the broccoli aptamer.
+<br>
+For more information, visit <a href="https://2020.igem.org/Team:Heidelberg/Pumilio_PPR#pumby">iGEM Heidelberg 2020</a></p>
 </html>