Difference between revisions of "Part:BBa K3425060"

 
 
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<partinfo>BBa_K3425060 short</partinfo>
 
<partinfo>BBa_K3425060 short</partinfo>
  
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BBa_K3425060 is a T7 polymerase regulated Juniper GFP substrate for Qβ replicase constructed from the weaker T7 RNA polymerase promoter, BBa_K3425035, the Qβ5' recognition sequence of Qβ replicase and RBS, BBa_K3425052, Juniper GFP for Type IIS iGEM standard assembly, BBa_K3425048, as well as the Qβ3' recognition sequence of Qβ replicase with terminator, BBa_K3425053.
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<b>QB recognition sites</b>
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<br>
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<b>Refereces</b>
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<br>[1]
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<br>Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
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<br><i>Note, that the authors declared the following competing financial interest(s): Y. Y., W. Z., and Q. W. have filed a patent on RNA replication system and its usage.</i>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:20, 28 October 2020


T7 polymerase regulated Juniper GFP substrate for Qβ replicase

BBa_K3425060 is a T7 polymerase regulated Juniper GFP substrate for Qβ replicase constructed from the weaker T7 RNA polymerase promoter, BBa_K3425035, the Qβ5' recognition sequence of Qβ replicase and RBS, BBa_K3425052, Juniper GFP for Type IIS iGEM standard assembly, BBa_K3425048, as well as the Qβ3' recognition sequence of Qβ replicase with terminator, BBa_K3425053.


QB recognition sites
Refereces
[1]
Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
Note, that the authors declared the following competing financial interest(s): Y. Y., W. Z., and Q. W. have filed a patent on RNA replication system and its usage.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 550
    Illegal XbaI site found at 83
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 550
    Illegal NheI site found at 859
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 550
    Illegal BglII site found at 86
    Illegal BamHI site found at 759
    Illegal XhoI site found at 90
    Illegal XhoI site found at 890
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 550
    Illegal XbaI site found at 83
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 550
    Illegal XbaI site found at 83
  • 1000
    COMPATIBLE WITH RFC[1000]