Difference between revisions of "Part:BBa K3657004"

Latest revision as of 03:15, 28 October 2020

RNA binding Pumby Protein - Motif AUAGAU

This Part is an example of a RNA-binding Pumby Protein, assembled with the Pumby Domains that are specific for the four RNA bases.

Adenine:BBa_K3657000

Guanine:BBa_K3657001

Uracil:BBa_K3657002

Cytosine:BBa_K3657003

Usage and Biology

Pumby Proteins are assembled by combining multiple RNA-base specific RNA binding Domains. They originate from the Pumilio Homology Domain (Drosophila Melanogaster), which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 184
Illegal XhoI site found at 224
Illegal XhoI site found at 449
Illegal XhoI site found at 634
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K3657004 short</partinfo>
 <html>
-This Part is an example of a Pumby Protein, assembled with the Pumby Domains that are specific for the four RNA bases.
+<br>
+This Part is an example of a RNA-binding Pumby Protein, assembled with the Pumby Domains that are specific for the four RNA bases.
 <p> Adenine:<a href="https://parts.igem.org/Part:BBa_K3657000">BBa_K3657000</a><p>
 Guanine:<a href="https://parts.igem.org/Part:BBa_K3657001">BBa_K3657001</a><p>
@@ Line 9: / Line 11: @@
 Cytosine:<a href="https://parts.igem.org/Part:BBa_K3657003">BBa_K3657003</a><p>
+</html>
 ===Usage and Biology===