Difference between revisions of "Part:BBa K3657012"
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In combination with part <a href="https://parts.igem.org/Part:BBa_K3657012">BBa_K3657012</a>, an assay was conducted, messuaring | In combination with part <a href="https://parts.igem.org/Part:BBa_K3657012">BBa_K3657012</a>, an assay was conducted, messuaring | ||
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Revision as of 03:15, 28 October 2020
RNA binding Pentatricopeptide Repeat Protein - Motif ACACAG
This Part is an example of a PPR Protein, assembled with the PPR Domains that are specific for the four RNA bases.
Adenine:BBa_K3657006
Guanine:BBa_K3657007
Uracil:BBa_K3657008
Cytosine:BBa_K3657009
It is an ON target PPR Protein for plasmides, containing the RBS BBa_B0032 because it binds to the RNA sequence ACACAG, which is part of the RBS.
It is an OFF target PPR Protein for the broccoli aptamer because it binds to the RNA sequence ACACAG, which cannot be found in the broccoli aptamers.
For more information, visit iGEM Heidelberg 2020
Usage and Biology
PPRs are originally found in Arabidopsis thaliana and can bind RNA. In chloroplasts, they take part in many post-translational regulation processes.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterisation
In combination with part <a href="https://parts.igem.org/Part:BBa_K3657012">BBa_K3657012</a>, an assay was conducted, messuaring
