Difference between revisions of "Part:BBa K3520019:Design"

 
 
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===Design Notes===
 
===Design Notes===
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The transcriptional units will be assembled using the Type IIS assembly standard.
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Each transcriptional unit is flanked with Sap I restrictions sites as well as a three nucleotide special sequence. Each part is digested with the appropriate enzyme and ligated in a one pot reaction creating a multi-transcriptional unit. The created multi-transcriptional unit is flanked with BsaI restriction sites.
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Latest revision as of 03:14, 28 October 2020


pHimarEm1+TUs of BcsA,B,C,D


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 16429
    Illegal suffix found in sequence at 9767
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 16429
    Illegal NheI site found at 10096
    Illegal SpeI site found at 9768
    Illegal PstI site found at 9782
    Illegal NotI site found at 9775
    Illegal NotI site found at 16435
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 16429
    Illegal BglII site found at 10418
    Illegal BglII site found at 12957
    Illegal BglII site found at 13616
    Illegal XhoI site found at 10053
    Illegal XhoI site found at 13254
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 16429
    Illegal suffix found in sequence at 9768
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 16429
    Illegal XbaI site found at 16444
    Illegal SpeI site found at 9768
    Illegal PstI site found at 9782
    Illegal NgoMIV site found at 11082
    Illegal AgeI site found at 12649
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The transcriptional units will be assembled using the Type IIS assembly standard.
Each transcriptional unit is flanked with Sap I restrictions sites as well as a three nucleotide special sequence. Each part is digested with the appropriate enzyme and ligated in a one pot reaction creating a multi-transcriptional unit. The created multi-transcriptional unit is flanked with BsaI restriction sites.



Source

αα

References