Difference between revisions of "Part:BBa K3520019"

Revision as of 03:07, 28 October 2020

pHimarEm1+TUs of BcsA,B,C,D

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found in sequence at 16429
Illegal suffix found in sequence at 9767
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 16429
Illegal NheI site found at 10096
Illegal SpeI site found at 9768
Illegal PstI site found at 9782
Illegal NotI site found at 9775
Illegal NotI site found at 16435
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 16429
Illegal BglII site found at 10418
Illegal BglII site found at 12957
Illegal BglII site found at 13616
Illegal XhoI site found at 10053
Illegal XhoI site found at 13254
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found in sequence at 16429
Illegal suffix found in sequence at 9768
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found in sequence at 16429
Illegal XbaI site found at 16444
Illegal SpeI site found at 9768
Illegal PstI site found at 9782
Illegal NgoMIV site found at 11082
Illegal AgeI site found at 12649
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K3520019 short</partinfo>
-[[File:T--Athens--Level_1_design.png|800px|thumb|center|Figure 1: Type IIS for level 1 Golden Gate assembly with SapI.]]
 <!-- Add more about the biology of this part here
@@ Line 16: / Line 14: @@
 The present plasmid is used to insert the Bcs operon genes (X54676.1) to a bacterium of the <i>Flavobacterium</i> genus through conjugation using an Escherichia coli strain for conjugation. Each gene has its own transcriptional unit that will allow their constitutive expression. These genes will all intergrated into the genome of the target <i>Flavobacterium</i> with the help of the Mariner transposon and then the Cellulose is produced by the recombinant bacteria.
+==Type IIS assembly design==
+[[File:T--Athens--Level_1_design.png|800px|thumb|center|Figure 1: Type IIS for level 1 Golden Gate assembly with SapI.]]
 <!-- -->