Difference between revisions of "Part:BBa K3657004"
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Pumby Proteins are assembled by combining multiple RNA-base specific RNA binding Domains. They originate from the Pumilio Homology Domain (Drosophila Melanogaster), which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus. | Pumby Proteins are assembled by combining multiple RNA-base specific RNA binding Domains. They originate from the Pumilio Homology Domain (Drosophila Melanogaster), which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus. | ||
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Revision as of 02:59, 28 October 2020
RNA binding Pumby Protein - Motif AUAGAU
This Part is an example of a RNA-binding Pumby Protein, assembled with the Pumby Domains that are specific for the four RNA bases.
Adenine:BBa_K3657000
Guanine:BBa_K3657001
Uracil:BBa_K3657002
Cytosine:BBa_K3657003
Usage and Biology
Pumby Proteins are assembled by combining multiple RNA-base specific RNA binding Domains. They originate from the Pumilio Homology Domain (Drosophila Melanogaster), which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus.
<img style="width:75%" src="
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Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 184
Illegal XhoI site found at 224
Illegal XhoI site found at 449
Illegal XhoI site found at 634 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]