Difference between revisions of "Part:BBa K3600015"

 
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<partinfo>BBa_K3600015 short</partinfo>
 
<partinfo>BBa_K3600015 short</partinfo>
  
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This cassette is the product of a two part (BBa_K3600008, BBa_K3600012) Golden Gate BsmBI assembly.  
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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This cassette will be integrated into the yeast genome to form a reporter strain that produces a fluorescent signal when the yeast cell is under environmental stress.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3600015 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3600006 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K3600015 parameters</partinfo>
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<partinfo>BBa_K3600006 parameters</partinfo>
 
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Revision as of 02:19, 28 October 2020


Lys2-YCF1pr-mScarleti cassette

This cassette is the product of a two part (BBa_K3600008, BBa_K3600012) Golden Gate BsmBI assembly.

Usage and Biology

This cassette will be integrated into the yeast genome to form a reporter strain that produces a fluorescent signal when the yeast cell is under environmental stress.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1184
    Illegal EcoRI site found at 1914
    Illegal XbaI site found at 1193
    Illegal XbaI site found at 1618
    Illegal XbaI site found at 2091
    Illegal SpeI site found at 3059
    Illegal PstI site found at 3068
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1184
    Illegal EcoRI site found at 1914
    Illegal SpeI site found at 3059
    Illegal PstI site found at 3068
    Illegal NotI site found at 1172
    Illegal NotI site found at 3078
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2661
    Illegal EcoRI site found at 1184
    Illegal EcoRI site found at 1914
    Illegal BglII site found at 1636
    Illegal BglII site found at 2532
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1184
    Illegal EcoRI site found at 1914
    Illegal XbaI site found at 1193
    Illegal XbaI site found at 1618
    Illegal XbaI site found at 2091
    Illegal SpeI site found at 3059
    Illegal PstI site found at 3068
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1184
    Illegal EcoRI site found at 1914
    Illegal XbaI site found at 1193
    Illegal XbaI site found at 1618
    Illegal XbaI site found at 2091
    Illegal SpeI site found at 3059
    Illegal PstI site found at 3068
    Illegal NgoMIV site found at 3016
  • 1000
    COMPATIBLE WITH RFC[1000]