Difference between revisions of "Part:BBa K3482018"

Latest revision as of 02:15, 28 October 2020

IPTG and heat-repressible miniColicin toxin

MiniColicin E2 version regulated by a heat-repressible termosensor and activited by IPTG under a pTac promotor

Usage and Biology

Colicin E2 is an endonuclease that cuts on both single and double-stranded DNA. It has no specific cutting site. Colicins are toxins that can be produced by E.coli and closely related bacteria.

We will use this part in a Kill switch with the IM2 protein, which provides immunity to Colicin E2.

Characterization

All the data concerning the characterization of that part can be found in the composite part : BBa_K3482017 .

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 539
Illegal PstI site found at 579
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 539
Illegal PstI site found at 579
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 539
Illegal PstI site found at 579
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 539
Illegal PstI site found at 579
Illegal AgeI site found at 155
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 MiniColicin E2 version regulated by a heat-repressible termosensor and activited by IPTG under a pTac promotor
 ===Usage and Biology===
-Colicin E2 is an endonuclease that cut on both single and double-stranded DNA. It has no specific cutting site. Colicin toxin are toxins that can be produced by E.coli and closely related bacteria.
+Colicin E2 is an endonuclease that cuts on both single and double-stranded DNA. It has no specific cutting site. Colicins are toxins that can be produced by E.coli and closely related bacteria.
-We will use this part in a Kill-switch with the IM2 immunity protein to Colicin E2.
+We will use this part in a Kill switch with the IM2 protein, which provides immunity to Colicin E2.
 ===Characterization===