Difference between revisions of "Part:BBa K3482018"

 
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<partinfo>BBa_K3482018 short</partinfo>
 
<partinfo>BBa_K3482018 short</partinfo>
  
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MiniColicin E2 version regulated by a heat-repressible termosensor and activited by IPTG under a pTac promotor
  
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===Usage and Biology===
 
===Usage and Biology===
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Colicin E2 is an endonuclease that cuts on both single and double-stranded DNA. It has no specific cutting site. Colicins are toxins that can be produced by E.coli and closely related bacteria.
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We will use this part in a Kill switch with the IM2 protein, which provides immunity to Colicin E2.
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===Characterization===
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All the data concerning the characterization of that part can be found in the composite part : <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3482017"> BBa_K3482017 </a></html>.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3482018 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3482018 SequenceAndFeatures</partinfo>

Latest revision as of 02:15, 28 October 2020


IPTG and heat-repressible miniColicin toxin

MiniColicin E2 version regulated by a heat-repressible termosensor and activited by IPTG under a pTac promotor

Usage and Biology

Colicin E2 is an endonuclease that cuts on both single and double-stranded DNA. It has no specific cutting site. Colicins are toxins that can be produced by E.coli and closely related bacteria.

We will use this part in a Kill switch with the IM2 protein, which provides immunity to Colicin E2.

Characterization

All the data concerning the characterization of that part can be found in the composite part : BBa_K3482017 .


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 539
    Illegal PstI site found at 579
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 539
    Illegal PstI site found at 579
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 539
    Illegal PstI site found at 579
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 539
    Illegal PstI site found at 579
    Illegal AgeI site found at 155
  • 1000
    COMPATIBLE WITH RFC[1000]