Difference between revisions of "Part:BBa K3520013"
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=Parts=
 
=Parts=
−
<b>Promoter:</b> ompA, a constituent Flavobacterial promoter.
+
<b>Promoter:</b> ompA, a constituent Flavobacterial promoter[1][2].
 
<br>
 
<br>
−
<b>RBS:</b> A Flavobacteria-specific RBS
+
<b>RBS:</b> A Flavobacteriia-specific RBS[1]
 
<br>
 
<br>
 
<b>CDS:</b> The bcsA gene.
 
<b>CDS:</b> The bcsA gene.
 
<br>
 
<br>
−
<b>Terminator:</b> A strong universal terminator.
+
<b>Terminator:</b> A strong universal double terminator.
  
 
<br><br>
 
<br><br>
 +
 +
=References=
 +
[1] Chen, S., Kaufman, M., Bagdasarian, M., Bates, A., & Walker, E. (2010). Development of an efficient expression system for Flavobacterium strains. Gene, 458(1-2), 1-10. doi: 10.1016/j.gene.2010.02.006 <br>
 +
[2] Chen, S., Bagdasarian, M., Kaufman, M., & Walker, E. (2006). Characterization of Strong Promoters from an Environmental Flavobacterium hibernum Strain by Using a Green Fluorescent Protein-Based Reporter System. Applied And Environmental Microbiology, 73(4), 1089-1100. doi: 10.1128/aem.01577-06
  
 
=Assembly=
 
=Assembly=
  
 
[[File:T--Athens--Level_0_design.png|800px|thumb|center|Figure 1: Type IIS design with RFC10 primers for TU parts.]]
 
[[File:T--Athens--Level_0_design.png|800px|thumb|center|Figure 1: Type IIS design with RFC10 primers for TU parts.]]

Latest revision as of 02:09, 28 October 2020


bcsA-Bacterial Cellulose Synthase A TU


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



This composite part is the transcriptional unit for BcsA. It was made by Type IIS Assembly. See the design tab for more information.

Parts

Promoter: ompA, a constituent Flavobacterial promoter[1][2].
RBS: A Flavobacteriia-specific RBS[1]
CDS: The bcsA gene.
Terminator: A strong universal double terminator.



References

[1] Chen, S., Kaufman, M., Bagdasarian, M., Bates, A., & Walker, E. (2010). Development of an efficient expression system for Flavobacterium strains. Gene, 458(1-2), 1-10. doi: 10.1016/j.gene.2010.02.006
[2] Chen, S., Bagdasarian, M., Kaufman, M., & Walker, E. (2006). Characterization of Strong Promoters from an Environmental Flavobacterium hibernum Strain by Using a Green Fluorescent Protein-Based Reporter System. Applied And Environmental Microbiology, 73(4), 1089-1100. doi: 10.1128/aem.01577-06

Assembly

Figure 1: Type IIS design with RFC10 primers for TU parts.