Difference between revisions of "Part:BBa K3629021"
 
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===Usage and Biology===
 
===Usage and Biology===
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mCherry is a monomeric red fluorescent protein that is derived from DsRed of <i>Discosoma.</i> mCherry's optimal excitation wavelength is 586nm and its optimal emission wavelength is 610nm (1). mCherry could be used to tag components and proteins within the cell which can then be visualized using fluorescence spectroscopy and microscopy.
  
 
===Design===
 
===Design===
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Below is the codon-optimized sequence of <i>Yarrowia lipolytica</i> mCherry for use in any expression construct. However, we have also made our own expression construct [https://parts.igem.org/Part:BBa_K3629025 BBa_K3629025], designed to allow for optimal expression of mCherry in <i>Yarrowia lipolytica</i> using the TEfin promoter [https://parts.igem.org/Part:BBa_K3629001 BBa_K3629001] and the XPR2 terminator [https://parts.igem.org/Part:BBa_K3629004 BBa_K3629004].
  
 
===Sequence and Features===
 
===Sequence and Features===
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===References===
 
===References===
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1. Lambert, T. (n.d.). MCherry at FPbase. Retrieved October 26, 2020, from https://www.fpbase.org/protein/mcherry/
  
 
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Latest revision as of 01:55, 28 October 2020


mCherry codon optimized for Yarrowia lipolytica

mCherry coding sequence codon-optimized for Yarrowia lipolytica.

Usage and Biology

mCherry is a monomeric red fluorescent protein that is derived from DsRed of Discosoma. mCherry's optimal excitation wavelength is 586nm and its optimal emission wavelength is 610nm (1). mCherry could be used to tag components and proteins within the cell which can then be visualized using fluorescence spectroscopy and microscopy.

Design

Below is the codon-optimized sequence of Yarrowia lipolytica mCherry for use in any expression construct. However, we have also made our own expression construct BBa_K3629025, designed to allow for optimal expression of mCherry in Yarrowia lipolytica using the TEfin promoter BBa_K3629001 and the XPR2 terminator BBa_K3629004.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

1. Lambert, T. (n.d.). MCherry at FPbase. Retrieved October 26, 2020, from https://www.fpbase.org/protein/mcherry/