Revision as of 01:38, 28 October 2020

Flavobacteria compatible Reflectin transcriptional unit with Signal Peptide

This composite parts is assembled with TYPEIIS Assembly and is consisted of the following subparts:

Promoter ompA + RBS for Flavobacteriia + Signal Peptide OPH + Codon optimised Reflectin + Double Terminator

codes for the BscC protein, a protein necessary for in vivo cellulose synthesis in Bacteria. It is codon optimised for Flavobacterium johnsoniae UW101

iGEM KU Istanbul 2020

This part was designed during the Partnership of iGEM Athens 2020 and iGEM KU Istanbul 2020. The latter team is creating a communication scheme between humans and biological cells by morphing cells into lasers. By this technology, they will be able to detect changes inside and around cells and tissues. These cell lasers can be employed in diagnostics and therapeutic purposes alongside as a high throughput method in basic research.

iGEM Athens 2020

iGEM Athens 2020 team during the project MORPHÆ works with Flavobacteriia to produce a non-cellular structurally coloured biomaterial which will require the secretion of a biomolecule that Flavobacteriia do not normally secrete. Our hypothesis is that the formed matrix will have a structure similar to that of the biofilm and thus, it will provide the material with macroscopically the same colouration properties as the biofilm.

So these two teams above, collaborated in a creative way and iGEM Athens designed a cloning experiment in which Flavobacteriia will express reflectin with a signal peptide which will translocate it to the outer membrane and GFP superfolde. As a result, the biolaser designed by iGEM KU Istanbul will be able to track genetically modified Flavobacteriia.