Difference between revisions of "Part:BBa K3408004"

 
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<partinfo>BBa_K3408004 short</partinfo>
 
<partinfo>BBa_K3408004 short</partinfo>
  
It is a protein which could suppress P<sub>CI</sub> so that our switch couldn't be transcribed without oxygen. It is also under the control of P<sub>nar</sub>.
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It is a protein which could suppress P<sub>CⅠ</sub> so that our switch RNA couldn't be transcribed without oxygen.  
  
We have optimized the CI protein on ThermoFisher SCIENTIFIC(https://www.thermofisher.com/cn/zh/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis/geneoptimizer.html) to use it better in our engineered bacteria.
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We designed it under the control of P<sub>nar</sub>.
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We have optimized the CⅠ protein on ThermoFisher SCIENTIFIC (https://www.thermofisher.com/cn/zh/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis/geneoptimizer.html) to use it better in our engineered bacteria.
  
 
https://2020.igem.org/wiki/images/4/43/T--NAU-CHINA--basic-parts3.png
 
https://2020.igem.org/wiki/images/4/43/T--NAU-CHINA--basic-parts3.png
 
https://2020.igem.org/wiki/images/5/5e/T--NAU-CHINA--basic-parts4.png
 
https://2020.igem.org/wiki/images/5/5e/T--NAU-CHINA--basic-parts4.png
  
The histograms show the percentage of sequence codons which fall into a certain quality class. The quality value of the most frequently used codon for a given amino acid in the desired expression system is set to 100, the remaining codons are scaled accordingly (see also Sharp, P.M., Li, W.H., Nucleic Acids Res. 15 (3),1987).
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&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp; &nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;Fig.1. Codon quality distribution        &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp; &nbsp; &nbsp;        Fig.2. Optimized codon quality distribution
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The histograms show the percentage of sequence codons which fall into a certain quality class. The quality value of the most frequently used codon for a given amino acid in the desired expression system is set to 100, the remaining codons are scaled accordingly (Sharp, P.M., Li, W.H., <i>Nucleic Acids Res</i>. 15 (3),1987).
  
 
https://2020.igem.org/wiki/images/b/bf/T--NAU-CHINA--basic-parts5.png
 
https://2020.igem.org/wiki/images/b/bf/T--NAU-CHINA--basic-parts5.png
 
https://2020.igem.org/wiki/images/7/77/T--NAU-CHINA--basic-parts6.png
 
https://2020.igem.org/wiki/images/7/77/T--NAU-CHINA--basic-parts6.png
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&nbsp; &nbsp; &nbsp; &nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;Fig.3. Codon quality plot              &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;          Fig.4. Optimized codon quality plot
  
 
The plots show the quality of the used codon at the indicated codon position.
 
The plots show the quality of the used codon at the indicated codon position.

Latest revision as of 01:35, 28 October 2020


Optimized CⅠ

It is a protein which could suppress PCⅠ so that our switch RNA couldn't be transcribed without oxygen.

We designed it under the control of Pnar.

We have optimized the CⅠ protein on ThermoFisher SCIENTIFIC (https://www.thermofisher.com/cn/zh/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis/geneoptimizer.html) to use it better in our engineered bacteria.

T--NAU-CHINA--basic-parts3.png T--NAU-CHINA--basic-parts4.png

                                 Fig.1. Codon quality distribution                                              Fig.2. Optimized codon quality distribution


The histograms show the percentage of sequence codons which fall into a certain quality class. The quality value of the most frequently used codon for a given amino acid in the desired expression system is set to 100, the remaining codons are scaled accordingly (Sharp, P.M., Li, W.H., Nucleic Acids Res. 15 (3),1987).

T--NAU-CHINA--basic-parts5.png T--NAU-CHINA--basic-parts6.png

                                 Fig.3. Codon quality plot                                                         Fig.4. Optimized codon quality plot

The plots show the quality of the used codon at the indicated codon position.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]