Difference between revisions of "Part:BBa K165028"
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<partinfo>BBa_K165028 short</partinfo> | <partinfo>BBa_K165028 short</partinfo> | ||
| − | This is the kozak sequence ligated to the Sin3 Repression domain. It can be used to build synthetic transcriptional repressing factors by ligating it to any DNA-binding domain, optionally a tandem set of fluorescent proteins, and to the [[Part:BBa_K165018|SV40 NLS +ADH1 terminator]] | + | This is the kozak sequence ligated to the Sin3 Repression domain. It can be used to build synthetic transcriptional repressing factors by ligating it to any DNA-binding domain, optionally a tandem set of fluorescent proteins to track expression, and to the [[Part:BBa_K165018|SV40 NLS +ADH1 terminator]] |
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Revision as of 06:22, 30 October 2008
Kozak + Sin3 Repression Domain
This is the kozak sequence ligated to the Sin3 Repression domain. It can be used to build synthetic transcriptional repressing factors by ligating it to any DNA-binding domain, optionally a tandem set of fluorescent proteins to track expression, and to the SV40 NLS +ADH1 terminator
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1775
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 946
Illegal SapI.rc site found at 1539