Difference between revisions of "Part:BBa K3408003"
 
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<partinfo>BBa_K3408003 short</partinfo>
 
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The switch RNA contains the coding sequence of the regulated gene. The upstream of the coding sequence is a hairpin structure which contains RBS, the start codon and a common 21nt-linker sequence. The 5' end of the hairpin provides a binding site for the trigger RNA. The binding of the trigger RNA to an unpaired toehold sequence of the switch hairpin allows for a branch migration process, exposing the start codon and ribosome binding site for translation initiation.
  
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It is the part of toehold switch which functions as the controller of suicide switch. It is activated by P<sub>CI</sub> when oxygen exists in wormcast.
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We used the toxin protein gene <i>mazF</i> as the suicide gene, when trigger RNA binds to the switch RNA, MazF protein will be expressed to kill bacteria.
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We hope our engineered bacteria not express MazF protein accidentally without having functioned yet, while we also couldn’t avoid the environment with oxygen when we cultivate them in the lab. So we added the Switch RNA to mazF, following the P<sub>CI</sub> downstream
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===Usage and Biology===
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Latest revision as of 01:30, 28 October 2020

Switch RNA(of toehold)

The switch RNA contains the coding sequence of the regulated gene. The upstream of the coding sequence is a hairpin structure which contains RBS, the start codon and a common 21nt-linker sequence. The 5' end of the hairpin provides a binding site for the trigger RNA. The binding of the trigger RNA to an unpaired toehold sequence of the switch hairpin allows for a branch migration process, exposing the start codon and ribosome binding site for translation initiation.

We used the toxin protein gene mazF as the suicide gene, when trigger RNA binds to the switch RNA, MazF protein will be expressed to kill bacteria.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 8
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]