Difference between revisions of "Part:BBa K2485000"

 
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<partinfo>BBa_K2485000 parameters</partinfo>
 
<partinfo>BBa_K2485000 parameters</partinfo>
 
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==Contribution ==
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*'''Group:''' [https://2020.igem.org/Team:SCU-WestChina SCU-WestChina iGEM team 2020]
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*'''Author:''' Mingyao Wang
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*'''Summary:''' function of SRPP4 cooperating with other subunits
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===Characterization ===
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Visualized expression with other subunits
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[[image: T--SCU-WestChina--improved_part1.png|600px|center|]]
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Fig1. The affinity of TbSRPPs for phospholipids depends on the head group and side chain, and influences particle size and distribution
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Function Evaluation:
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(1) Its expression can be upregulated by abiotic stress and promote water-stress tolerance in A. thaliana which indicate its function of abiotic stress resistance.(shown in Fig1)
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(2) Its intracellular localization in N. benthamiana protoplasts is almost completely the same with TbSRPP2/5.
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For one thing, after testing its function of stability resistance and assembly with other subunits in lipid droplet, it’s revealed that SRPP4 is essential for rubber particle integrity with SRPP5.
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For another, TbSRPP3–5 (and TbREF) facilitate the formation of a rubber biosynthesis complex, then TbSRPP4 and TbSRPP2 cooperate to move lipid.
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[[image: T--SCU-WestChina--improved_part2.png|600px|center|]]
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Fig2. Expression levels changes of SRPP4 under abiotic stress conditions.
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[[image: T--SCU-WestChina--improved_part3.png|600px|center|]]
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Fig3. Intracellular localization of TbSRPP4 in N. benthamiana protoplasts.
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[[image: T--SCU-WestChina--improved_part4.png|600px|center|]]
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Fig4. Intracellular localization of TbSRPP4 in N. benthamiana leaf epidermis cells.
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Refrence
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[1]Laibach, N., Schmidl, S., Müller, B., Bergmann, M., Prüfer, D., & Schulze Gronover, C. (2018). Small rubber particle proteins from Taraxacum brevicorniculatum promote stress tolerance and influence the size and distribution of lipid droplets and artificial poly(cis -1,4-isoprene) bodies. The Plant Journal, 93(6), 1045–1061. doi:10.1111/tpj.13829

Latest revision as of 01:22, 28 October 2020


SRPP4 Taraxacum brevicorniculatum

This makes a protein for latex production in the dandelion species Taraxacum brevicorniculatum.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 318



Contribution


Characterization

Visualized expression with other subunits

T--SCU-WestChina--improved part1.png

Fig1. The affinity of TbSRPPs for phospholipids depends on the head group and side chain, and influences particle size and distribution

Function Evaluation: (1) Its expression can be upregulated by abiotic stress and promote water-stress tolerance in A. thaliana which indicate its function of abiotic stress resistance.(shown in Fig1) (2) Its intracellular localization in N. benthamiana protoplasts is almost completely the same with TbSRPP2/5. For one thing, after testing its function of stability resistance and assembly with other subunits in lipid droplet, it’s revealed that SRPP4 is essential for rubber particle integrity with SRPP5. For another, TbSRPP3–5 (and TbREF) facilitate the formation of a rubber biosynthesis complex, then TbSRPP4 and TbSRPP2 cooperate to move lipid.

T--SCU-WestChina--improved part2.png

Fig2. Expression levels changes of SRPP4 under abiotic stress conditions.

T--SCU-WestChina--improved part3.png

Fig3. Intracellular localization of TbSRPP4 in N. benthamiana protoplasts.

T--SCU-WestChina--improved part4.png

Fig4. Intracellular localization of TbSRPP4 in N. benthamiana leaf epidermis cells.

Refrence [1]Laibach, N., Schmidl, S., Müller, B., Bergmann, M., Prüfer, D., & Schulze Gronover, C. (2018). Small rubber particle proteins from Taraxacum brevicorniculatum promote stress tolerance and influence the size and distribution of lipid droplets and artificial poly(cis -1,4-isoprene) bodies. The Plant Journal, 93(6), 1045–1061. doi:10.1111/tpj.13829