Difference between revisions of "Part:BBa K3408002"
 
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<partinfo>BBa_k3408002 short</partinfo>
 
<partinfo>BBa_k3408002 short</partinfo>
  
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It is the part of toehold switch which functions as the key to relieve switch RNA and activate downstream circuit.  
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Trigger RNA is a short RNA sequence that can bind to the switch RNA to initiate translation of downstream gene.
  
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We modifed our trigger RNA with some bps in the 5’ terminal to increase its stability.
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In our gene circuit, we need high stability and long half-life of trigger RNA. Based on literature, we found that 5' hairpin could improve stability of mRNA. By imitating this design, we added 5’ hairpin to trigger RNA to meet our need.
  
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In our project we would use the trigger NRA to release mazF when switch-mazF is expressed in wormcast. So our engineered bacterium could commit suicide.  
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In our project we would use trigger RNA to release the expression of <i>mazF</i> when switch-mazF is expressed in excrement. So our engineered bacteria could commit suicide.  
  
  
 
https://2020.igem.org/wiki/images/4/48/T--NAU-CHINA--basic-parts2.png
 
https://2020.igem.org/wiki/images/4/48/T--NAU-CHINA--basic-parts2.png
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<span style="text-align:center; display:inline-block;">Fig.3. Effect of different Synthetic 5′Secondary Structures to half-life of mRNA(Carrier T A, et al., <i>Biotechnology Progress</i>. 2010)</span>
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<span style="text-align:center; display:inline-block;">Fig.1. Effect of different Synthetic 5' Secondary Structures to half-life of mRNA(Carrier T A, et al., <i>Biotechnology Progress</i>. 2010)</span>
  
 
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Latest revision as of 01:19, 28 October 2020

Trigger RNA(of toehold)

Trigger RNA is a short RNA sequence that can bind to the switch RNA to initiate translation of downstream gene.

In our gene circuit, we need high stability and long half-life of trigger RNA. Based on literature, we found that 5' hairpin could improve stability of mRNA. By imitating this design, we added 5’ hairpin to trigger RNA to meet our need.

In our project we would use trigger RNA to release the expression of mazF when switch-mazF is expressed in excrement. So our engineered bacteria could commit suicide.


T--NAU-CHINA--basic-parts2.png Fig.1. Effect of different Synthetic 5' Secondary Structures to half-life of mRNA(Carrier T A, et al., Biotechnology Progress. 2010)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 110
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]