Difference between revisions of "Part:BBa K3408002"
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| − | <span style="text-align:center; display:inline-block;">Fig.1. Effect of different Synthetic | + | <span style="text-align:center; display:inline-block;">Fig.1. Effect of different Synthetic 5' Secondary Structures to half-life of mRNA(Carrier T A, et al., <i>Biotechnology Progress</i>. 2010)</span> |
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Revision as of 01:12, 28 October 2020
Trigger RNA(of toehold)
Trigger RNA is a short RNA sequence that can bind to the switch RNA to initiate translation of downstream gene.
In our gene circuit, we need high stability and long half-life of trigger RNA. Based on literature, we found that 5' hairpin could improve stability of mRNA. By imitating this design, we added 5’ hairpin to trigger RNA to meet our need.
In our project we would use trigger RNA to release the expression of mazF when switch-mazF is expressed in wormcast. So our engineered bacteria could commit suicide.
Fig.1. Effect of different Synthetic 5' Secondary Structures to half-life of mRNA(Carrier T A, et al., Biotechnology Progress. 2010)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 110
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]