Difference between revisions of "Part:BBa K3408001"
 
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It is the enzyme which could fix lead efficiently in a middle pH environment.  
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Phytase is a kind of phosphate-solubilizing enzyme, which can hydrolyze phytate to release phosphate.
  
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Because of the limitation of pH, we have to choose the phosphate-solubilizing enzyme which could work efficiently <b>between the pH from pH6.0 to pH7.0</b>. And we have found the Phy(ycD) whose <b>optimum reaction pH is pH6.5</b>. Thus, we added it into the anaerobic regulation circuit, following the P<sub>nar</sub>.
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Considering the limitation of pH, we chose the phytase which could work efficiently under pH 6.0 to pH 7.0. We have found the <i>phy(ycD)</i> from <i>Bacillus subtilis</i> YCD whose optimum reaction pH is 6.5(Fig.1). Thus, we constructed <i>phy(ycD)</i> gene into our engineered bacteria.
  
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https://2020.igem.org/wiki/images/a/a0/T--NAU-CHINA--Basic-part-2.png
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<img src="https://2020.igem.org/wiki/images/a/a0/T--NAU-CHINA--Basic-part-2.png" style="width:50%"/>
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<span style="text-align:center; display:inline-block;">Fig.2. Phy (ycD) activity changes with temperature(Wang Xi, et al., <i>Applied and Environmental Biology</i>. 2014)</span>
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<span style="text-align:center; display:inline-block;">Fig.1. Effects of pH on the phytase activity (Wang Xi, et al., <i>Applied and Environmental Biology</i>. 2014)</span>
  
  
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According to the paper, we could see its <b>optimum reaction temperature is 45℃</b>, Km is 0.213mmol/L and Vmax is 5.55U/mg.
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According to the paper, we could see its <b>optimum reaction pH is 6.5</b>.
  
 
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Latest revision as of 01:08, 28 October 2020

Phy(ycD)(Phytase)

Phytase is a kind of phosphate-solubilizing enzyme, which can hydrolyze phytate to release phosphate.

Considering the limitation of pH, we chose the phytase which could work efficiently under pH 6.0 to pH 7.0. We have found the phy(ycD) from Bacillus subtilis YCD whose optimum reaction pH is 6.5(Fig.1). Thus, we constructed phy(ycD) gene into our engineered bacteria.

Fig.1. Effects of pH on the phytase activity (Wang Xi, et al., Applied and Environmental Biology. 2014)


According to the paper, we could see its optimum reaction pH is 6.5.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 359
  • 1000
    COMPATIBLE WITH RFC[1000]