Difference between revisions of "Part:BBa K3593010"

 
 
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<partinfo>BBa_K3593010 short</partinfo>
 
<partinfo>BBa_K3593010 short</partinfo>
  
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This fragment of antibody derives from the monoclonal antibody that can specifically target &#945;-amatoxin exist in the samples with relatively low sensitivity.
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This ssDNA can be immobilized on the control line region of test strip. It is complementary with the best 1 aptamer conjugated to a reporter Au nanoparticle, thus reporting a red color to verify the function of lateral flow construction
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=Sequence and features=
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<partinfo>BBa_K3593010 SequenceAndFeatures</partinfo>
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=Characterisation of this part=
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==Design==
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Besides aptamers, we have some short single stranded DNA known as 'probes', which binds to other single stranded DNA. BBa_K3593010 is one of those so-called 'probes' in our design, and play important roles in our hardware, in which it binds and immobilizes the aptamer. Its 5' end is conjugated to biotin[1] to bind with streptavidin.
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=References=
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1.Jauset-Rubio M, Svobodová M, Mairal T, et al. Aptamer Lateral Flow Assays for Ultrasensitive Detection of β-Conglutin Combining Recombinase Polymerase Amplification and Tailed Primers. Anal Chem. 2016;88(21):10701-10709. doi:10.1021/acs.analchem.6b03256
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
  
 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3593010 SequenceAndFeatures</partinfo>
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Latest revision as of 00:59, 28 October 2020


ssDNA, capture probe of control line in method without RPA

This ssDNA can be immobilized on the control line region of test strip. It is complementary with the best 1 aptamer conjugated to a reporter Au nanoparticle, thus reporting a red color to verify the function of lateral flow construction

Sequence and features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterisation of this part

Design

Besides aptamers, we have some short single stranded DNA known as 'probes', which binds to other single stranded DNA. BBa_K3593010 is one of those so-called 'probes' in our design, and play important roles in our hardware, in which it binds and immobilizes the aptamer. Its 5' end is conjugated to biotin[1] to bind with streptavidin.

References

1.Jauset-Rubio M, Svobodová M, Mairal T, et al. Aptamer Lateral Flow Assays for Ultrasensitive Detection of β-Conglutin Combining Recombinase Polymerase Amplification and Tailed Primers. Anal Chem. 2016;88(21):10701-10709. doi:10.1021/acs.analchem.6b03256