Difference between revisions of "Part:BBa K3425061"
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<partinfo>BBa_K3425061 short</partinfo> | <partinfo>BBa_K3425061 short</partinfo> | ||
− | + | BBa K3425061 is a Constitutive expression construct of Qβ replicase, and is constructed from the constitutive promoter, BBa_J23108, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, β-subunit of Qβ replicase with cleavable degradation tag, BBa_K3425051, as well as the double terminator BBa_B0015. | |
− | tends to mutate in high copy number | + | From observations have can it be stated that this construct tends to mutate in high copy number plasmids |
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Revision as of 00:41, 28 October 2020
Constitutive expression construct of Qβ replicase
BBa K3425061 is a Constitutive expression construct of Qβ replicase, and is constructed from the constitutive promoter, BBa_J23108, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, β-subunit of Qβ replicase with cleavable degradation tag, BBa_K3425051, as well as the double terminator BBa_B0015.
From observations have can it be stated that this construct tends to mutate in high copy number plasmids
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1377
Illegal XhoI site found at 885
Illegal XhoI site found at 1051 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]