Difference between revisions of "Part:BBa K3657021"

 
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<partinfo>BBa_K3657021 short</partinfo>
 
<partinfo>BBa_K3657021 short</partinfo>
  
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This part is a fusion protein. It consists of a PPR complex that binds GUAGAG RNA loop and the Ribonuclease A.
  
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===Usage and Biology===
 
  
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===Usage and Biology===
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Ribonuclease A (<a href="https://parts.igem.org/Part:BBa_K3657019">BBa_K3657019</a>) is a unspecific highly active endonuclease.
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Single PPR subunits bind to specific ribonucleotides. Concatenation of the subunits forms a protein that binds a specific RNA sequence.
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In that way this construct can deliver Ribonuclease A to a specific RNA. It was planned to be used to bind to the following Broccoli modifications: <a href="https://parts.igem.org/Part:BBa_K3657017">BBa_K3657017</a>, <a href="https://parts.igem.org/Part:BBa_K3657018">BBa_K3657018</a>. More information can be found here: <a href="https://2020.igem.org/Team:Heidelberg/Pumilio_PPR">iGEM Heidelberg 2020.</a>
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<p>Due to the COVID pandemic we were not able to experimentally examine performance of this part.</p>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3657021 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3657021 SequenceAndFeatures</partinfo>

Latest revision as of 00:37, 28 October 2020


RNA Motif GUAGAG targeted PPR - Ribonuclease A Complex

This part is a fusion protein. It consists of a PPR complex that binds GUAGAG RNA loop and the Ribonuclease A.


Usage and Biology

Ribonuclease A (BBa_K3657019) is a unspecific highly active endonuclease.

Single PPR subunits bind to specific ribonucleotides. Concatenation of the subunits forms a protein that binds a specific RNA sequence.

In that way this construct can deliver Ribonuclease A to a specific RNA. It was planned to be used to bind to the following Broccoli modifications: BBa_K3657017, BBa_K3657018. More information can be found here: iGEM Heidelberg 2020.

Due to the COVID pandemic we were not able to experimentally examine performance of this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]