Difference between revisions of "Part:BBa K3475008"

Revision as of 00:29, 28 October 2020

Prm+ RBS GFP Ter

GFP activated by cI

Usage and Biology

FIGURE 4. The relative fluorescence of HrtR- (E.coli-pSB1K3-HrtO-cI-pA1c-R0051-mRFP-I12007-GFP), HrtR+ (E.coli-pHSB-cI-pA1c-R0051-mRFP-I12007-GFP) and WT (E.coli-wildtype).
Measured by excitation wavelength of 475 nm, emission wavelength of 515 nm for GFP; and by excitation wavelength of 588 nm, emission wavelength of 635 nm for mRFP. Relative fluorescence is calculated by the formula below, LB medium as blank.As shown in the figure, when heme is sufficient (simulated by KO of HrtR), the expression of GFP (promoter: I12007) is activated and the expression of RFP (promoter: R0051) is repressed; when heme lacks, the expression of GFP (promoter: I12007) is repressed and the expression of RFP (promoter: R0051) is activated. This can to some extent prove the engineering success of the connection between heme biosensor system and bilateral ARG system.

FIGURE 5. Calculating formula.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 738

@@ Line 9: / Line 9: @@
 <div style="text-align:center">[[Image:T--Tongji_China--202_6.jpg | 750px]]</div>
 <p style="width:600px; margin-left:150px; margin-bottom:60px;
-text-align:justify "><em><strong>FIGURE 4.</strong> '''The relative fluorescence of WT (E.coli-wildtype), J23110 (E.coli-pSB1C3-K608010), HrtO (E.coli-pUC-GW-HrtO-GFP) and I12007 (E.coli-pSB1C3-I12007-GFP-pSB1K3-HrtO-cI).'''<br/> Measured by excitation wavelength of 475 nm, emission wavelength of 515 nm.  Relative fluorescence is calculated by the formula below, LB medium as blank. Relative fluorescence is shown in logarithmic axis, 2 to the power of n.
+text-align:justify "><em><strong>FIGURE 4.</strong> '''The relative fluorescence of HrtR- (E.coli-pSB1K3-HrtO-cI-pA1c-R0051-mRFP-I12007-GFP), HrtR+ (E.coli-pHSB-cI-pA1c-R0051-mRFP-I12007-GFP) and WT (E.coli-wildtype).'''<br/> Measured by excitation wavelength of 475 nm, emission wavelength of 515 nm for GFP; and by excitation wavelength of 588 nm, emission wavelength of 635 nm for mRFP. Relative fluorescence is calculated by the formula below, LB medium as blank.As shown in the figure, when heme is sufficient (simulated by KO of HrtR), the expression of GFP (promoter: I12007) is activated and the expression of RFP (promoter: R0051) is repressed; when heme lacks, the expression of GFP (promoter: I12007) is repressed and the expression of RFP (promoter: R0051) is activated. This can to some extent prove the engineering success of the connection between heme biosensor system and bilateral ARG system.
- </em> </p>
+ </em> </p>
 <div style="text-align:center">[[Image:T--Tongji_China--202_t.jpg | 750px]]</div>
 <p style="width:600px; margin-left:150px; margin-bottom:60px;
-text-align:justify "><em><strong>FIGURE 5.</strong> '''Calculating formula.'''<br/> As shown in the figure, the expression strength of I12007 is about 8% of HrtO, under the activation of cI (promoter: HrtO), showing 92% of loss during signal transformation from HBS to BAS.
+text-align:justify "><em><strong>FIGURE 5.</strong> '''Calculating formula.'''<br/>
   </em> </p>