Difference between revisions of "Part:BBa K3657000"
Line 3: Line 3:
 
<partinfo>BBa_K3657000 short</partinfo>
 
<partinfo>BBa_K3657000 short</partinfo>
  
 +
<html>
 +
<p>
 
The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificallity is due to a variation in the amino acid in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, being able to bind a sequence of up to 18 RNA nucleotides.  
 
The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificallity is due to a variation in the amino acid in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, being able to bind a sequence of up to 18 RNA nucleotides.  
−
 
+
</p>
−
 
+
<br>
−
This part is the Adenine binding Pumby Domain.  
+
<p>
−
 
+
This part is the Adenine binding Pumby Domain.
−
 
+
</p>
−
Combined with the other Pumby Domains (BBa_K3657001, BBa_K3657002, BBa_K3657003) it can be assembled to a Pumby Protein, binding a specific RNA sequence (see the compotitive parts: BBa_K3657004 and BBa_K3657005)
+
<br>
−
 
+
<p>
 +
It can be assembled to a Pumby Protein when combined with the other Pumby Domains: <p> Guanine:<a href="https://parts.igem.org/Part:BBa_K3657001">BBa_K3657001</a><p>
 +
Uracil:<a href="https://parts.igem.org/Part:BBa_K3657002">BBa_K3657002</a><p>
 +
Cytosine:<a href="https://parts.igem.org/Part:BBa_K3657003">BBa_K3657003</a><p>
 +
<br>
 +
See also the compositive parts: <a href="https://parts.igem.org/Part:BBa_K3657004">BBa_K3657004</a>, <a href="https://parts.igem.org/Part:BBa_K3657005">BBa_K3657005</a>)
 +
<p>
 +
</html>
 
===Usage and Biology===
 
===Usage and Biology===
  
 
Pumby Domains are engeneered from the Pumilio Homology Domain (Drosophila Melanogaster), which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus.
 
Pumby Domains are engeneered from the Pumilio Homology Domain (Drosophila Melanogaster), which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus.
−
 
  
  

Revision as of 00:26, 28 October 2020


RNA binding Pumilio Homology Domain (Adenine)

The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificallity is due to a variation in the amino acid in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, being able to bind a sequence of up to 18 RNA nucleotides.


This part is the Adenine binding Pumby Domain.


It can be assembled to a Pumby Protein when combined with the other Pumby Domains:

Guanine:BBa_K3657001

Uracil:BBa_K3657002

Cytosine:BBa_K3657003


See also the compositive parts: BBa_K3657004, BBa_K3657005)

Usage and Biology

Pumby Domains are engeneered from the Pumilio Homology Domain (Drosophila Melanogaster), which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]