Difference between revisions of "Part:BBa K3657002"

Revision as of 00:24, 28 October 2020

RNA binding Pumilio Homology Domain (Uracil)

The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificallity is due to a variation in the amino acid in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, being able to bind a sequence of up to 18 RNA nucleotides.

This part is the Uracil binding Pumby Domain.

It can be assembled to a Pumby Protein when combined with the other Pumby Domains:

Adenine:BBa_K3657000

Guanine:BBa_K3657001

Cytosine:BBa_K3657003

See also the compositive parts: BBa_K3657004, BBa_K3657005)

Usage and Biology

Pumby Domains are engeneered from the Pumilio Homology Domain (Drosophila Melanogaster), which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 70
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 14: / Line 14: @@
 <p>
 It can be assembled to a Pumby Protein when combined with the other Pumby Domains: <p> Adenine:<a href="https://parts.igem.org/Part:BBa_K3657000">BBa_K3657000</a><p>
-Cytosine:<a href="https://parts.igem.org/Part:BBa_K3657002">BBa_K3657002</a><p>
+Guanine:<a href="https://parts.igem.org/Part:BBa_K3657001">BBa_K3657001</a><p>
-Guanine:<a href="https://parts.igem.org/Part:BBa_K3657003">BBa_K3657003</a><p>
+Cytosine:<a href="https://parts.igem.org/Part:BBa_K3657003">BBa_K3657003</a><p>
 <br>
 See also the compositive parts: <a href="https://parts.igem.org/Part:BBa_K3657004">BBa_K3657004</a>, <a href="https://parts.igem.org/Part:BBa_K3657005">BBa_K3657005</a>)